The response of aminopeptidases of Phaseolus vulgaris to drought depends on the developmental stage of the leaves

摘要: Aminopeptidases, together with other proteases, execute and regulate the total specifically limited protein breakdown involved in plant physiology, raising possibility of their involvement response to drought. We have identified, leaves Phaseolus vulgaris L., five aminopeptidases (E.C.3.4.11) whose levels activity changed when three week old plants were subjected First, second third trifoliate investigated separately. The first identified then isolated using ion exchange chromatography leaf extracts. Three, named PvAP1, PvAP2 PvAP4, are metallo broad substrate specificity, active against substrates conjugated alanine lysine. Two others, PvAP3 PvAP5, apparently serine aminopeptidases, former phenylalanine leucine, latter characterised by narrow specificity phenylalanine. Their apparent molecular weights range from ∼37 kDa ∼80 kDa. Levels individual both watered drought stressed shown depend on age leaves. In they generally highest young, very low older, leaves, exception PvAP5. Drought initiated an almost general increase activities, although different extents, PvAP4 PvAP5 young Thus, such studies it is necessary investigate effects separately ages order elucidate complex probably specific roles common bean