Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches

摘要: Osteoarticular pathologies very often require an implementation therapy to favor regeneration processes of bone, cartilage and/or tendons. Clinical approaches performed on osteoarticular complications in dogs constitute ideal model for human clinical translational applications. The adipose-derived mesenchymal stem cells (ASCs) have already been used accelerate and facilitate the regenerative process. ASCs can be maintained vitro they differentiated osteocytes or chondrocytes offering a good tool cell replacement therapies veterinary medicine. Although ACSs easily obtained from adipose tissue, amplification process is usually by time consuming successive passages. In this work, we use canine using Bioreactor device under GMP culture conditions that produces minimum 30 million within 2 weeks. This method provides rapid aseptic production sufficient with potential further We show plasma rich growth factors (PRGF) treatment positively contributes viability proliferation into caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) scaffolds. biomaterial does not need additional modifications cASCs attachment proliferation. Here propose framework based combination may contribute increase therapeutical capability PRGF compatible biomaterials bone connective tissue regeneration.