Patient-derived xenografts recapitulate molecular features of human uveal melanomas
作者： Cécile Laurent , David Gentien , Sophie Piperno-Neumann , Fariba Némati , André Nicolas
DOI: 10.1016/J.MOLONC.2013.02.004
关键词: GNA11 、 Mutation 、 GNAQ 、 GNAS complex locus 、 Gene expression profiling 、 BAP1 、 Biology 、 Cancer research 、 In vivo 、 Melanoma 、 Molecular medicine 、 Genetics 、 General Medicine
摘要: We have previously developed a new method for the development and maintenance of uveal melanoma (UM) xenografts in immunodeficient mice. Here, we compare genetic profiles primary tumors to their corresponding that been passaged over time. The study included sixteen UMs at very early (P1), (P4), late (P9) vivo passages. were analyzed mutation status GNAQ, GNA11, GNAS, GNA15, BAP1, BRAF, chromosomal copy number alterations using Affymetrix GeneChip® Genome-Wide Human SNP6.0 arrays, gene expression Exon 1.0 ST BAP1 mRNA protein expression, MAPK pathway Reverse Phase Protein Arrays (RPPA). UM accurately recapitulated features human they exhibited stability course maintenance. Our technique establishing maintaining as xenograft mice exhibit high degree conservation between multiple passages vivo. These models therefore constitute valuable preclinical tool drug screening UM.
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