Agrobacterium mediated transformation of Tunisian Cucumis melo cv. maazoun
作者： Awatef Rhimi , Michel Hernould , Mohamed Boussaid
关键词: Kanamycin 、 Biology 、 Transformation (genetics) 、 Agrobacterium tumefaciens 、 Agrobacterium 、 Molecular biology 、 Botany 、 Murashige and Skoog medium 、 Cucumis 、 Kinetin 、 Explant culture
摘要: Transgenic Cucumis melo cv. Maazoun containing the neomycin phosphotransferase II (NPT II) chimeric gene conferring resistance to kanamycin were obtained from cotyledons explants inoculated with Agrobacterium tumefaciens (GV3101) that contained binary vector plasmid pADI. Transformed shoots on Murashige and Skoog medium supplemented 1.50 mgl-1 IAA, 0.10mgl-1 BAP, 0.01 NAA 6 kinetin. Transformants selected by using only 100 of 4 days pre-culture. Putative transformants confirmed for transgene insertion through polymerase chain reaction (PCR) analysis. From explants, 6.66% produced transgenic shoots.
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sci-hub.st 参考文章(20)
F. A. Tang, K. Alpert, G. G. Sarmento, Z. K. Punja, Factors influencing Agrobacterium tumefaciens mediated transformation and expression of kanamycin resistance in pickling cucumber Plant Cell Tissue and Organ Culture. ,vol. 31, pp. 185- 193 ,(1992) , 10.1007/BF00036222
MIREIA BORDAS, CONSUELO MONTESINOS, MERCEDES DABAUZA, AURORA SALVADOR, LUIS ANTONIO ROIG, RAMON SERRANO, VICENTE MORENO, Transfer of the yeast salt tolerance gene HAL1 to Cucumis melo L. cultivars and in vitro evaluation of salt tolerance. Transgenic Research. ,vol. 6, pp. 41- 50 ,(1997) , 10.1023/A:1018453032336
Ricardo Ayub, Monique Guis, Mohamed Ben Amor, Laurent Gillot, Jean-Paul Roustan, Alain Latché, Mondher Bouzayen, Jean-Claude Pech, Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits. Nature Biotechnology. ,vol. 14, pp. 862- 866 ,(1996) , 10.1038/NBT0796-862
Yoko Akasaka-Kennedy, Ken-o Tomita, Hiroshi Ezura, Efficient plant regeneration and Agrobacterium-mediated transformation via somatic embryogenesis in melon (Cucumis melo L.) Plant Science. ,vol. 166, pp. 763- 769 ,(2004) , 10.1016/J.PLANTSCI.2003.11.020
P. Trionfetti Nisini, G. Colla, E. Granati, O. Temperini, P. Crinò, F. Saccardo, Rootstock resistance to fusarium wilt and effect on fruit yield and quality of two muskmelon cultivars Scientia Horticulturae. ,vol. 93, pp. 281- 288 ,(2002) , 10.1016/S0304-4238(01)00335-1
Isabelle Debeaujon, Michel Branchard, Induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (Cucumis melo L.). Plant Cell Reports. ,vol. 12, pp. 37- 40 ,(1992) , 10.1007/BF00232420
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Monique Guis, Mohamed Ben Amor, Alain Latché, Jean-Claude Pech, Jean-Paul Roustan, A reliable system for the transformation of cantaloupe charentais melon (Cucumis melo L. var. cantalupensis) leading to a majority of diploid regenerants Scientia Horticulturae. ,vol. 84, pp. 91- 99 ,(2000) , 10.1016/S0304-4238(99)00101-6
Guowei Fang, Rebecca Grumet, Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. Plant Cell Reports. ,vol. 9, pp. 160- 164 ,(1990) , 10.1007/BF00232095
PaulaP. Chee, Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants. Plant Cell Reports. ,vol. 9, pp. 245- 248 ,(1990) , 10.1007/BF00232293