Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants.

摘要: Cotyledons of cucumber seedlings (Cucumis sativus L. cv. Poinsett 76) were co-cultivated with disarmed Agrobacterium strain C58Z707. The contained the Agrobacterium-derived binary vector plasmid pGA482, its T-DNA region contains a plant expressible bacterial derived neomycin phosphotransferase II (NPT II) gene which upon transfer, genome integration, and expression in tissues confers resistance to antibiotic kanamycin. After growth inoculated cotyledon sections on selective medium containing 100 mg/l kanamycin, transformed embryogenic calli obtained followed by development embryos regeneration. Transformed R0 R1 plants appeared normal tested positive for NPT enzyme activity. Genomic DNAs isolated from all showed hybridization characteristic 2.0 kb (BamHI HindIII) gene-containing fragment. These results show that Agrobscterium-mediated transfer system regeneration via somatic embryogenesis is an effective method genetic material into species belonging family Cucurbitaceae.