Oligomerization of oncoprotein p53.

摘要: Cellular phosphoprotein p53, which seems to be a multifunctional protein, may assigned different structural subclasses. Recently established immortalized or transformed cell lines that overexpress p53 allowed us perform detailed analysis of the quaternary structure p53. By means sucrose density gradient centrifugation, we found in simian virus 40-transformed cells addition high-molecular-weight T-p53 complexes, low-molecular-weight forms. The level complexes within seemed determined by intracellular concentration However, presence uncomplexed T antigen and indicated an appropriate modification at least one two proteins appears necessary for complex formation. Using monoclonal antibodies distinguish between (i) associated with heat shock (ii) apparently free form, from always normal cells, however, was mainly Pulse-labeling experiments revealed oligomerization is very rapid process. Monomeric forms could detected only 2 min pulse-labeling were rapidly converted stable, oligomers. Furthermore, our data indicate correlation occurrence transformation state cell.