Immobilization studies of an engineered arginine-tryptophan-rich peptide on a silicone surface with antimicrobial and antibiofilm activity.
作者： Kaiyang Lim , Ray Rong Yuan Chua , Rathi Saravanan , Anindya Basu , Biswajit Mishra
DOI: 10.1021/AM401629P
关键词: Nanotechnology 、 Antimicrobial surface 、 Combinatorial chemistry 、 Antimicrobial 、 Antimicrobial peptides 、 Cytotoxicity 、 Materials science 、 Infrared spectroscopy 、 Membrane 、 Silicone 、 Peptide
摘要: With the rapid rise of antibiotic-resistant-device-associated infections, there has been increasing demand for an antimicrobial biomedical surface. Synthetic peptides that have excellent bactericidal potency and negligible cytotoxicity are promising targets immobilization on these target surfaces. An engineered arginine–tryptophan-rich peptide (CWR11) was developed, which displayed potent activity against a broad spectrum microbes via membrane disruption, possessed salt resistance properties. A tethering platform subsequently developed to tether CWR11 onto model polymethylsiloxane (PDMS) surface using simple robust strategy. Surface characterization assays such as attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron (XPS), energy-dispersive (EDX) confirmed successful grafting chemically treated PDMS The immobilized concentra...
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