Analysis of the mechanism(s) of metaphase I arrest in maturing mouse oocytes

摘要: Fully grown mouse oocytes are normally competent to progress from prophase I metaphase II without interruption. However, growing initially become only partially undergo meiotic maturation. Meiotic maturation in these does not beyond I. In contrast the of most strains mice, strain LT/Sv mice arrested at even when they fully grown. The initiation oocyte is correlated with an increase p34cdc2 kinase activity that continues rise until transition into anaphase a decrease activity. This study demonstrated arrest both and sustained elevation fact, continued during time decreased. maturing oocytes, some, but all, cyclin B, regulatory protein associated p34cdc2, became degraded entered contrast, amount B present I-arrested it was being normal progressing II. These results suggest progression meiosis groups because sustained, least part, by restricted degradation B.(ABSTRACT TRUNCATED AT 250 WORDS)