Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard

作者: Burkhard Malorny , Jeffrey Hoorfar , Cornelia Bunge , Reiner Helmuth

DOI: 10.1128/AEM.69.1.290-296.2003

关键词: Gel electrophoresisGeneSalmonella speciesChromosomal dnaSalmonellaPrimer (molecular biology)DNABiologyDetection limitMolecular biology

摘要: … The exclusivity tests with 47 non-Salmonella strains gave unspecific PCR fragments (data not shown). Primer set P1-P2 (oriC) (25) produced multiple, non-target-sized fragments of 0.2 …

参考文章(21)
T. Humphrey, Public-health aspects of Salmonella infection. Salmonella in domestic animals.. pp. 245- 263 ,(2000) , 10.1079/9780851992617.0245
A J Bäumler, F Heffron, R Reissbrodt, Rapid detection of Salmonella enterica with primers specific for iroB. Journal of Clinical Microbiology. ,vol. 35, pp. 1224- 1230 ,(1997) , 10.1128/JCM.35.5.1224-1230.1997
Clifford Wray, A Wray, Salmonella in Domestic Animals ,(2000)
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
David J Wallace, Thomas Van Gilder, Sue Shallow, Terry Fiorentino, Suzanne D Segler, Kirk E Smith, Beletshachew Shiferaw, Ruth Etzel, Wallace E Garthright, Frederick J Angulo, Foodnet Working Group, None, Incidence of Foodborne Illnesses Reported by the Foodborne Diseases Active Surveillance Network (FoodNet)-1997 Journal of Food Protection. ,vol. 63, pp. 807- 809 ,(2000) , 10.4315/0362-028X-63.6.807
Michael McClelland, Kenneth E. Sanderson, John Spieth, Sandra W. Clifton, Phil Latreille, Laura Courtney, Steffen Porwollik, Johar Ali, Mike Dante, Feiyu Du, Shunfang Hou, Dan Layman, Shawn Leonard, Christine Nguyen, Kelsi Scott, Andrea Holmes, Neenu Grewal, Elizabeth Mulvaney, Ellen Ryan, Hui Sun, Liliana Florea, Webb Miller, Tamberlyn Stoneking, Michael Nhan, Robert Waterston, Richard K. Wilson, Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 Nature. ,vol. 413, pp. 852- 856 ,(2001) , 10.1038/35101614
Burkhard Malorny, Andreas Schroeter, Cornelia Bunge, Bernhard Hoog, Antje Steinbeck, Reiner Helmuth, Evaluation of molecular typing methods for Salmonella enterica serovar Typhimurium DT104 isolated in Germany from healthy pigs. Veterinary Research. ,vol. 32, pp. 119- 129 ,(2001) , 10.1051/VETRES:2001116
M. N. Widjojoatmodjo, A. C. Fluit, R. Torensma, B. H. I. Keller, J. Verhoef, Evaluation of the magnetic immuno PCR assay for rapid detection of Salmonella. European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 10, pp. 935- 938 ,(1991) , 10.1007/BF02005447
S. Aabo, O.F. Rasmussen, L. Roseen, P.D. Sørensen, J.E. Olsen, Salmonella identification by the polymerase chain reaction Molecular and Cellular Probes. ,vol. 7, pp. 171- 178 ,(1994) , 10.1006/MCPR.1993.1026