Simplified procedures for detection of amplified DNA using fluorescent label incorporation and reverse probing
作者： Alison J. Woolford , Jeremy W. Dale
DOI: 10.1111/J.1574-6968.1992.TB05587.X
关键词: Molecular biology 、 DNA 、 Hybridization probe 、 Bacteriology 、 Primer dimer 、 Fluorescein 、 Biology 、 Molecular probe 、 Applications of PCR 、 Polymerase chain reaction 、 Genetics 、 Microbiology
摘要: Conventional methods of detecting polymerase chain reaction (PCR) products require equipment and expertise which may not be available in diagnostic bacteriology laboratories, especially developing countries. To this end we have examined other product detection, including fluorescein-12-dUTP incorporation during PCR amplification, reverse probing, where the is used as probe a scaled down hybridization with fixed capture consisting fragment entirely internal to sequence product. These techniques shown sensitivities 20 fg purified mycobacterial DNA, corresponds approximately five cells.
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