Microsphere-mediated protein delivery into cells.
作者： Rosario M. Sanchez-Martin , Lois Alexander , Mathilde Muzerelle , Juan M. Cardenas-Maestre , Anestis Tsakiridis
关键词: Enzyme 、 lac operon 、 Nanoparticle 、 Carrier system 、 Chemistry 、 Green fluorescent protein 、 Fusion protein 、 Coupling (electronics) 、 Transporter 、 Biophysics 、 Biochemistry
摘要: Delivering the goods: By coupling proteins to varyingly sized polymeric microspheres, it is possible deliver them cells in an easy and effective way. For this study a fluorescent protein (EGFP) functional enzyme (β-galactosidase) were coupled these particles. Evaluation of cellular uptake after “beadfection” shows that functionality activity not adversely affected through carrier system; their structure retained.
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