Polymerizable Fluorescein Derivatives: Synthesis of Fluorescent Particles and Their Cellular Uptake

摘要: Fluorescent particles are used for a diverse number of biochemical assays including intracellular imaging, cellular tracking, as well detection variety biomolecules. They typically prepared by postpolymerization conjugations dyes onto preformed particles. Herein we report the synthesis aminomethyl-functionalized fluorescent via and application polymerizable fluorescein monomers. These monomers allowed high controllable fluorophore loading into particles, resulting in enhanced fluorescence properties comparison with more commonly carboxyfluorescein conjugated Furthermore, were rapidly taken up cells fluorescence. The herein presented results demonstrate advantages dye polymerization contrast conventional conjugation strategies particle generation applications life sciences.