Palladium-mediated bioorthogonal conjugation of dual-functionalised nanoparticles and their cellular delivery
作者： Frank Thielbeer , Sunay V. Chankeshwara , Emma M. V. Johansson , Neil Norouzi , Mark Bradley
DOI: 10.1039/C2SC20706K
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摘要: Nanoparticles have gained considerable significance in the life sciences due to their ability be internalised by living cells and relative ease with which they can functionalised cargos ranging from molecular sensors biomacromolecules. However, scope of available bioconjugation methods is limited new bioorthogonal are much sought after. Herein, we present dual (HO)2B/H2N-polymeric nanoparticles conjugated via amide bond formation and/or Pd-mediated Suzuki–Miyaura cross-coupling a chemoselective manner. These dual-functionalised particles were found efficiently taken up mammalian without toxicity successfully employed cellular delivery intracellular release “turn-on” probe thereby demonstrating potential use for chemical biology applications.
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Frank Thielbeer, Ken Donaldson, Mark Bradley, Zeta Potential Mediated Reaction Monitoring on Nano and Microparticles Bioconjugate Chemistry. ,vol. 22, pp. 144- 150 ,(2011) , 10.1021/BC1005015
Rosario M. Sanchez-Martin, Lois Alexander, Mathilde Muzerelle, Juan M. Cardenas-Maestre, Anestis Tsakiridis, Joshua M. Brickman, Mark Bradley, Microsphere-mediated protein delivery into cells. ChemBioChem. ,vol. 10, pp. 1453- 1456 ,(2009) , 10.1002/CBIC.200900136
Rahimi M. Yusop, Asier Unciti-Broceta, Emma M. V. Johansson, Rosario M. Sánchez-Martín, Mark Bradley, Palladium-mediated intracellular chemistry Nature Chemistry. ,vol. 3, pp. 239- 243 ,(2011) , 10.1038/NCHEM.981
Sneha S. Kelkar, Theresa M. Reineke, Theranostics: Combining Imaging and Therapy Bioconjugate Chemistry. ,vol. 22, pp. 1879- 1903 ,(2011) , 10.1021/BC200151Q
Frank Thielbeer, Sunay V. Chankeshwara, Mark Bradley, Polymerizable Fluorescein Derivatives: Synthesis of Fluorescent Particles and Their Cellular Uptake Biomacromolecules. ,vol. 12, pp. 4386- 4391 ,(2011) , 10.1021/BM201394K
Juan Manuel Cardenas-Maestre, Sonia Panadero-Fajardo, Ana Maria Perez-Lopez, Rosario M. Sanchez-Martin, Sulfhydryl reactive microspheres for the efficient delivery of thiolated bioactive cargoes Journal of Materials Chemistry. ,vol. 21, pp. 12735- 12743 ,(2011) , 10.1039/C1JM11948F
T.C. Yih, M. Al-Fandi, Engineered nanoparticles as precise drug delivery systems Journal of Cellular Biochemistry. ,vol. 97, pp. 1184- 1190 ,(2006) , 10.1002/JCB.20796
Nicolas Vogel, Christoph P. Hauser, Kilian Schuller, Katharina Landfester, Clemens K. Weiss, Accurate Elemental Analysis of Metal-Containing Polymer Latexes Using ICP-Optical Emission Spectrometry Macromolecular Chemistry and Physics. ,vol. 211, pp. 1355- 1368 ,(2010) , 10.1002/MACP.201000036
Kwangmeyung Kim, Misu Lee, Hyungkyu Park, Jong-Ho Kim, Sungwon Kim, Hesson Chung, Kuiwon Choi, In-San Kim, Baik L. Seong, Ick Chan Kwon, Cell-permeable and biocompatible polymeric nanoparticles for apoptosis imaging. Journal of the American Chemical Society. ,vol. 128, pp. 3490- 3491 ,(2006) , 10.1021/JA057712F
Asier Unciti-Broceta, Juan José Díaz-Mochón, Rosario M Sanchez-Martin, Mark Bradley, None, The Use of Solid Supports to Generate Nucleic Acid Carriers Accounts of Chemical Research. ,vol. 45, pp. 1140- 1152 ,(2012) , 10.1021/AR200263C