A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.

摘要: The alteration in the surface of endothelial cells (EC) response to cytokines is likely be great importance regulation cell migration and thereby evolution inflammatory processes. We have generated three mAb against cytokine inducible Ag on EC. Whereas 1.2B6 6.5B5 were found react with ELAM-1 ICAM-1, respectively, 1.4C3 reacted a novel molecule that showed different pattern expression from or ICAM-1 after stimulation EC by TNF, IL-1, LPS. Like ELAM-1, was minimally expressed resting EC, whereas moderately expressed. After LPS, maximal 4 6 h, 10 24 h. duration intermediate between more prolonged TNF than IL-1 similar dose varying concentrations required 10-fold higher concentration for half (5 ng/ml compared 0.5 ng/ml). Of Ag, only enhanced IFN-gamma. SDS-PAGE under reducing conditions migrate as single band relative molecular mass approximately 95 kDa. adds our understanding kinetics will useful studying activation. Furthermore, may an important role leukocyte-EC interactions.