Repetitive Hypershear Activates and Sensitizes Platelets in a Dose-Dependent Manner

摘要: Implantation of mechanical circulatory support (MCS) devices-ventricular assist devices and the total artificial heart-has emerged as a vital therapy for advanced end-stage heart failure. Unfortunately, MCS patients face requirement life-long antiplatelet anticoagulant to combat thrombotic complications resulting from dynamic supraphysiologic shear stress conditions associated with such devices, whose effect on platelet activation is poorly understood. We developed syringe-capillary viscometer-the "platelet hammer"-that repeatedly exposed platelets average stresses up 1000 dyne/cm(2) short 25 ms. Platelet state was measured using modified prothrombinase assay, morphological changes analyzed scanning electron microscopy. observed an increase in post-high rate, or sensitization, accumulation (SA), product exposure time. A significant beyond SA 1500 dyne-s/cm(2) , marked pseudopod length visible . Utility hammer extends studies other shear-dependent pathologies, may development approaches enhance safety effectiveness objective antithrombotic pharmacotherapy management.