Transcription from a bacteriophage T4 middle promoter using T4 motA protein and phage-modified RNA polymerase.
作者： D.M. Hinton
DOI: 10.1016/S0021-9258(18)55233-9
关键词: Consensus sequence 、 RNA polymerase 、 RNA 、 Molecular biology 、 Bacteriophage 、 Promoter 、 Transcription (biology) 、 Gene expression 、 Biology 、 Gene
摘要: The bacteriophage T4 motA protein is required for transcription from middle promoters. These promoters, which contain the Escherichia coli promoter consensus sequence at -10 region (TATAAT) but a unique centered -30 ((a/t)(a/t)TGCTT(t/c)A) (Guild, N., Gayle, M., Sweeney, R., Hollingsworth, T., Modeer, and Gold, L. (1988) J. Mol. Biol. 199, 241-258), become active about 2 min after infection, time when host RNA polymerase has been modified by phage proteins. This paper shows that binds to in vitro addition of allows this T4-modified polymerase. gene was cloned into multicopy plasmid complemented mutants vivo. MotA protein, partially purified cells containing motA+ plasmid, specifically retarded electrophoretic mobility an oligomer located 195 bases upstream uvsX (PuvsX). isolated infected during expression supported PuvsX only fractions were added. In contrast, unmodified catalyzed synthesis minor amounts PuvsX, not dependent. Thus, system described here provides basis detailed study factors needed regulate expression.
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