Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya.

摘要: Abstract Following an outbreak of trypanosomosis in horses on a farm Kenya, 18 trypanosome isolates were collected from the infected animals over period one and half years cryopreserved for characterization. The characterization was done basis morphology using Giemsa-stained blood buffy coat smears, infectivity to mice, recombinant DNA hybridization, chromosome separation by orthogonal field alternation gel electrophoresis (OFAGE). Morphologically, all identified as belonging subgenus Nannomonas, total 16 out grew mice. Using hybridization technique, further classified ‘savannah’ type Trypanosoma congolense. Furthermore, OFAGE, carried six clones derived different isolates, exhibited profile characteristic T. congolense, type. However, there differences number positions medium-sized minichromosomes indicating diversity serodemes within isolates. Hence infecting trypanosomes this disease type, comprised several or strains.