Production of lysophospholipids and free fatty acids by a sarcolemmal fraction from canine myocardium.
作者： Kenneth Owens , David C. Pang , William B. Weglicki
DOI: 10.1016/0006-291X(79)90639-9
关键词: Sarcolemma 、 Lysophospholipids 、 Phosphatidylethanolamine 、 Fatty acid 、 Biochemistry 、 Endogeny 、 Chemistry 、 Hydrolysis 、 Calcium 、 Chromatography 、 Incubation
摘要: Abstract The potential for injury of myocardial sarcolemma by endogenous lipases was studied. sarcolemmal fraction incubated 30 min under conditions found optimal hydrolysis exogenous phosphatidylethanolamine (5 mM calcium, pH 7.0, 37°C). Incubation the increased significantly level total free fatty acids (14.1 to 31.1 nmoles/mg protein, P
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sci-hub.se 参考文章(10)
Grant R. Bartlett, Phosphorus Assay in Column Chromatography Journal of Biological Chemistry. ,vol. 234, pp. 466- 468 ,(1959) , 10.1016/S0021-9258(18)70226-3
William R. Morrison, Lloyd M. Smith, Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol Journal of Lipid Research. ,vol. 5, pp. 600- 608 ,(1964) , 10.1016/S0022-2275(20)40190-7
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
W.B. Weglicki, M. Waite, P. Sisson, S.B. Shohet, Myocardial phospholipase a of microsomal and mitochondrial fractions Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 231, pp. 512- 519 ,(1971) , 10.1016/0005-2760(71)90119-6
Richard Franson, Moseley Waite, William Weglicki, Phospholipase A activity of lysosomes of rat myocardial tissue Biochemistry. ,vol. 11, pp. 472- 476 ,(1972) , 10.1021/BI00753A028
R Franson, Phospholipase A activity of highly enriched preparations of cardiac sarcolemma from hamster and dog Journal of Molecular and Cellular Cardiology. ,vol. 10, pp. 921- 930 ,(1978) , 10.1016/0022-2828(78)90338-3
J LAMERS, W HULSMANN, Inhibition of (Na++K+)-stimulated ATPase of heart by fatty acids Journal of Molecular and Cellular Cardiology. ,vol. 9, pp. 343- 346 ,(1977) , 10.1016/S0022-2828(77)80039-4
W Weglicki, Association of phospholipase A with a myocardial membrane preparation containing the (Na+ + K+)-Mg2+-ATPase Journal of Molecular and Cellular Cardiology. ,vol. 4, pp. 195- 201 ,(1972) , 10.1016/0022-2828(72)90057-0
David C. Pang, William B. Weglicki, Cardiac sarcolemma of the hamster. Enrichment of the (Na+ + K+)-ATPase Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 465, pp. 411- 414 ,(1977) , 10.1016/0005-2736(77)90091-8
John D. Turner, George Rouser, Precise quantitative determination of human blood lipids by thin-layer and triethylaminoethyl-cellulose column chromatography Analytical Biochemistry. ,vol. 38, pp. 423- 436 ,(1970) , 10.1016/0003-2697(70)90467-7