Cardiac sarcolemma of the hamster. Enrichment of the (Na+ + K+)-ATPase
作者： David C. Pang , William B. Weglicki
DOI: 10.1016/0005-2736(77)90091-8
关键词: Myofibril 、 Sarcolemma 、 Hamster 、 Homogenization (biology) 、 Gradient centrifugation 、 Na+/K+-ATPase 、 Biology 、 Continuous density 、 Mitochondrion 、 Chromatography 、 Biophysics 、 Cell biology 、 Biochemistry
摘要: Abstract Cardiac sarcolemma was prepared from normal hamsters using gentle homogenization, extraction with 0.6 M KCl and continuous density gradient centrifugation. The final fraction exhibited high (Na + K )-ATPase activity (24 μmol/mg per h) contained minimal contamination mitochondria, myofibrils lysosomes.
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