Studies on the synthesis of short-chain geranyl esters catalysed by Fusarium oxysporum esterase in organic solvents
作者： H Stamatis , P Christakopoulos , D Kekos , B.J Macris , F.N Kolisis
DOI: 10.1016/S1381-1177(98)00003-4
关键词: Organic chemistry 、 Chemistry 、 Water activity 、 Esterase 、 Enzyme 、 Organic solvent 、 Catalysis 、 Hexane 、 Fusarium oxysporum 、 Geraniol
摘要: Abstract A novel esterase isolated from Fusarium oxysporum was investigated for the synthesis of short-chain esters geraniol by alcoholysis and direct esterification reactions in organic solvents. The enzyme used as a dried powder (i.e., not immobilized). reaction parameters affecting behavior such nature solvent acyl donor, concentration substrates water activity system were studied. High yields (80–90%) obtained both approaches (alcoholysis esterification) at low values ( w =0.11) n -hexane. retain its catalytic even after fifth reuse -hexane =0.11, demonstrating stability efficiency under conditions this study.
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sci-hub.se 参考文章(25)
H.F. de Castro, P.C. de Oliveira, E.B. Pereira, Evaluation of different approaches for lipase catalysed synthesis of citronellyl acetate Biotechnology Letters. ,vol. 19, pp. 229- 232 ,(1997) , 10.1023/A:1018349406159
Flavor chemistry : trends and developments ChemInform. ,vol. 21, ,(1989) , 10.1021/BK-1989-0388
A Zaks, A M Klibanov, The effect of water on enzyme action in organic media. Journal of Biological Chemistry. ,vol. 263, pp. 8017- 8021 ,(1988) , 10.1016/S0021-9258(18)68435-2
N. A. Turner, D. B. Duchateau, E. N. Vulfson, Effect of hydration on thermostability of serine esterases Biotechnology Letters. ,vol. 17, pp. 371- 376 ,(1995) , 10.1007/BF00130792
A Klibanov, Enzymatic catalysis in anhydrous organic solvents Trends in Biochemical Sciences. ,vol. 14, pp. 141- 144 ,(1989) , 10.1016/0968-0004(89)90146-1
William F Fett, Hervé C Gérard, Robert A Moreau, Stanley F Osman, Love E Jones, None, Cutinase production by Streptomyces spp. Current Microbiology. ,vol. 25, pp. 165- 171 ,(1992) , 10.1007/BF01571025
Paul A. Claon, Casimir C. Akoh, Effect of reaction parameters on SP435 lipase-catalyzed synthesis of citronellyl acetate in organic solvent Enzyme and Microbial Technology. ,vol. 16, pp. 835- 838 ,(1994) , 10.1016/0141-0229(94)90056-6
M. Karra-Chaabouni, S. Pulvin, D. Touraud, D. Thomas, Enzymatic synthesis of geraniol esters in a solvent-free system by lipases Biotechnology Letters. ,vol. 18, pp. 1083- 1088 ,(1996) , 10.1007/BF00129736
Paul A. Claon, Casimir C. Akoh, Enzymatic synthesis of geraniol and citronellol esters by direct esterification in n-hexane Biotechnology Letters. ,vol. 15, pp. 1211- 1216 ,(1993) , 10.1007/BF00130299
Peter J. Halling, Thermodynamic predictions for biocatalysis in nonconventional media: theory, tests, and recommendations for experimental design and analysis. Enzyme and Microbial Technology. ,vol. 16, pp. 178- 206 ,(1994) , 10.1016/0141-0229(94)90043-4