Organization and function of stress fibers in cells in vitro and in situ. A review.

摘要: The purpose of this review is to present the tremendous body research on stress fibers, which has grown exceedingly rapidly in last 7 or 8 years, due immunofluorescent techniques, with both a technical and functional perspective. first section primarily chronology innovations have enabled better observation characterization fibers. This also reviews numerous contractile-associated proteins, actin-binding regulator other proteins shown localize fibers characteristic distribution. second discusses wide variety roles for that been set forth, including cell spreading, adhesion, locomotion, contraction, isometric surface compartmentalization, differentiation, transformation, tumorigenicity, morphogenesis. In order help interpret significance many purported it important ask whether vitro are pure artifacts exist cells situ. demonstrates fiber fundamentally light microscopic term, avoid confusion microfilament bundle-containing structures seen electron microscope, such as circumferential bundles, contractile rings, microvilli, microspikes, rootlet structures, necessary establish criteria identification Finally, using these criteria, paper presents two recently introduced models exhibit tissues: fibroblast, called scleroblast fish scale, endothelial avian mammalian vasculature.