Assessment of colorectal cancer prognosis through nuclear morphometry.

摘要: BACKGROUND Due to the fact that different biological parameters play a key role in colonic malignant behavior, with nuclear morphometry being prognostic marker many malignancies, then predictive approaches colorectal cancer (CRC) carried out on histologically well-defined groups may prevent interpretative errors. Subsequently, present study, CRC patients were screened according morphometric features of tumor cell nuclei, using an accurate histotechnical approach, analyze their clinical evolution Dukes' stratification. MATERIALS AND METHODS A total 66 cases grouped classification (5 y follow-up). The perimeter, area, and shape factor 50 interphase carcinoma nuclei recorded through microphotographs obtained from each subject. Nuclei boundaries drawn by electronic pencil examined computerized system. Data submitted variance analysis, multi-regression model compared results. RESULTS sample was made up 44 males (66.67%) 22 females (33.33%) aged 59.7 +/- 6 old. Forty-nine (74.24%) classified as stage B, 17 (25.76%) C. Nuclear homogeneity confirmed analysis variance. (mean SD): area (3.17 1.74), perimeter (6.72 1.83), (0.82 0.03). multiple logistic regression showed C subjects had higher risk developing worse than those at B (P < 0.02), independent sex age. CONCLUSIONS remains best predictor evolution. Although nucleomorphometric suitability is still controversial, this technique has become additional tool establish prognosis.