Prognostic Values of microRNAs in Colorectal Cancer

摘要: The functions of non-coding microRNAs (miRNAs) in tumorigenesis are just beginning to emerge. Previous studies from our laboratory have identified a number miRNAs that were deregulated colon cancer cell lines due the deletion p53 tumor suppressor gene. In this study, vivo significance some these was further evaluated using colorectal clinical samples. Ten (hsa-let-7b, hsa-let-7g, hsa-miR-15b, hsa-miR-181b, hsa-miR-191, hsa-miR-200c, hsa-miR-26a, hsa-miR-27a, hsa-miR-30a-5p and hsa-miR-30c) for their potential prognostic value patients. Forty eight snap frozen samples (24 24 paired normal patient samples) with detailed follow-up information selected. expression levels 10 quantified via qRT-PCR analysis. statistical markers disease prognosis two tailed Wilcoxon test. A Kaplan-Meier survival curve generated followed by performing Logrank Among ten miRNAs, hsa-miR-15b (p = 0.0278), hsa-miR-181b 0.0002), hsa-miR-191 0.0264) hsa-miR-200c 0.0017) significantly over-expressed tumors compared analysis indicated associated 0.0122). patients (n 15) higher had shorter time (median 26 months) 9) lower 38 months). Sequencing revealed 0.0098) 0.0322) strongly mutation status Some may function as oncogenes over-expression tumors. be novel factor cancer.