The recovery of added nematode eggs from horse and sheep faeces by three methods

作者: Antonio Bosco , Maria Paola Maurelli , Davide Ianniello , Maria Elena Morgoglione , Alessandra Amadesi

DOI: 10.1186/S12917-017-1326-7

关键词: BiologyVeterinary medicineAnthelminticFecesEggs per gramNematode eggHorseNematode

摘要: Nematode infections in horses are widespread across the world. Increasing levels of anthelmintic resistance, reported worldwide equine parasites, have led to creation programs for control nematodes based on faecal egg counts (FEC). To improve nematode counting samples and establish whether matrix faeces or eggs affect counts, analytical sensitivity, accuracy precision Mini-FLOTAC (combined with Fill-FLOTAC), McMaster Cornell-Wisconsin techniques were compared. Known numbers extracted from ovine added free give 10, 50, 200 500 per gram (EPG) faeces. The significantly underestimated showed a low revealing 100% sensitivity only concentrations greater than EPG. EPG values detected by did not differ expected at any level density. combined Fill-FLOTAC which provides an accurate method weighing without need balance filtering out debris, could be used FEC farm as well laboratory.

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