3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum
作者： Ramli B. Mohd. Isa , A.B. Sipat
DOI: 10.1016/0006-291X(82)91852-6
关键词: Activator (genetics) 、 Biochemistry 、 Enzyme activator 、 Enzyme assay 、 Hydroxymethylglutaryl-CoA reductase 、 Dithiothreitol 、 Chromatography 、 Reductase 、 Ammonium 、 Chemistry 、 Hevea brasiliensis 、 Biophysics 、 Cell biology 、 Molecular biology
摘要: Abstract The effect of the C-serum (the cytosol) on activity 3-hydroxy-3-methylglutaryl CoA reductase in latex Hevea brasiliensis was investigated. Depending clone from which obtained, found to depress or activate have little enzyme activity. Boiling however, resulted a consistent activation all clones examined. Optimal obtained with 20 μl boiled C-serum. Dialysis EDTA (40 mM) treatment did not diminish effect. Although essential, dithiothreitol complemented and optimal concentration 10 mM. Trypsin digestion complete loss activator salted out by ammonium sulphate at 25 – 100% saturation. Hevein had no
elsevier.com
sci-hub.se 参考文章(7)
VICTOR W. RODWELL, JEFFREY L. NORDSTROM, JONATHAN J. MITSCHELEN, Regulation of HMG-CoA Reductase Advances in lipid research. ,vol. 14, pp. 1- 74 ,(1976) , 10.1016/B978-0-12-024914-5.50008-5
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A.I. McMullen, Thiols of low molecular weight in Hevea brasiliensis latex Biochimica et Biophysica Acta. ,vol. 41, pp. 152- 154 ,(1960) , 10.1016/0006-3002(60)90383-8
Zafarul H. Beg, David W. Allmann, David M. Gibson, Modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity with cAMP and with protein fractions of rat liver cytosol Biochemical and Biophysical Research Communications. ,vol. 54, pp. 1362- 1369 ,(1973) , 10.1016/0006-291X(73)91137-6
David J. Shapiro, Jeffrey L. Nordstrom, Jonathan J. Mitschelen, Victor W. Rodwell, Robert T. Schimke, Micro assay for 3-hdyroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts Biochimica et Biophysica Acta. ,vol. 370, pp. 369- 377 ,(1974) , 10.1016/0005-2744(74)90098-9
B. L. Archer, The proteins of Hevea brasiliensis latex. 4. Isolation and characterization of crystalline hevein* Biochemical Journal. ,vol. 75, pp. 236- 240 ,(1960) , 10.1042/BJ0750236
Stanley Goldfarb, Henry C. Pitot, Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Journal of Lipid Research. ,vol. 12, pp. 512- 515 ,(1971) , 10.1016/S0022-2275(20)39504-3