Optimized application of surface‐enhanced laser desorption/ionization time‐of‐flight MS to differentiate Francisella tularensis at the level of subspecies and individual strains
作者： Eric Seibold , Ralf Bogumil , Sonja Vorderwülbecke , Sascha Al Dahouk , Arno Buckendahl
DOI: 10.1111/J.1574-695X.2007.00216.X
关键词: Francisella tularensis 、 Microbiology 、 Mass spectrometry 、 Biology 、 Subspecies 、 Typing 、 Strain (biology) 、 Genetics 、 Surface-enhanced laser desorption/ionization 、 Francisella 、 Tularemia
摘要: Francisella tularensis, the causative agent of tularaemia, is a potential bioterrorism. The phenotypic discrimination closely related F. tularensis subspecies and individual strains with traditional methods difficult time consuming, often producing ambiguous results. Surface-enhanced laser desorption/ionization time-of-flight MS (SELDI-TOF MS) was used in this study to discriminate different species genus Francisella. We tested 18 including at least one representative each species/subspecies on four types chromatographic chip surfaces. Multivariate analysis (hierarchical clustering principal component analysis) allowed grouping according their designated subspecies. Furthermore, single within could be discriminated.
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