MALDI-TOF mass spectrometry of bacteria
作者： Jackson O. Lay
DOI: 10.1002/MAS.10003
关键词: Biomolecule 、 Proteomics 、 Proteome 、 Virulence 、 Mass spectrometry 、 Bacteria 、 Chemistry 、 Matrix-assisted laser desorption/ionization 、 Analytical chemistry 、 Biochemistry 、 DNA 、 General Biochemistry, Genetics and Molecular Biology 、 Spectroscopy 、 Condensed matter physics
摘要: The development of MALDI-TOF mass spectrometry methods for the characterization bacteria is reviewed and discussed. general use MALDI large biomolecules led directly to obvious applications involving analysis isolated bacterial proteins. More surprising was observation that could be applied crude cellular fractions or suspensions resulting data from such complex mixtures provide evidence chemotaxonomic classification. Versatility rapidity rapid a number bacteria. Examples some covered in this review are RNA DNA, detection recombinant proteins, targeted unknown proteomics, virulence markers, very at genus, species, strain level. demonstrated capability taxonomic classification level, using unprocessed cells, opens possibility similar approaches may contribute significantly fulfilling emerging needs near real-time
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