Mutagenicity, Genotoxicity, and Estrogenic Activity of River Porewaters

摘要: We investigated mutagenicity, genotoxicity, and estrogenic activity in the porewaters of two river basins southern Italy that had different features. Three samples from each site were collected seasons 7 sites for a total 21 samples. Mutagenicity was measured with Ames test without metabolic activation (S9) using Salmonella typhimurium TA98 TA100 strains. Genotoxicity tests: one involved chromophore detected DNA damage Escherichia coli PQ37 (SOS chromotest), other micronuclei formation root cells Vicia faba. Estrogenic yeast-based estrogen receptor assay an MCF-7 cell-based, estrogen-sensitive proliferation assay. also applied chemical analyses to detect alkylphenols, pesticides, natural synthetic hormones, heavy metals. The both sediments showed mutagenic/genotoxic on V. faba test, latter S9 liver fraction. SOS chromotest not sufficiently sensitive genotoxicity porewaters, but repair system E. activated presence mix. Good correlations found between mutagenicity/genotoxicity concentration cadmium copper. This study assessed concentrations some bioavailable pollutants porewater overall effects multiple contributed these basin thus increasing our understanding environmental consequences polluted aquatic ecosystems.