Comparison Between New Wild Plant Trifolium repens and Vicia faba on their Sensitivity in Detecting the Genotoxic Potential of Heavy Metal Solutions and Heavy Metal-Contaminated Soils

作者: Nicolas Manier , Annabelle Deram , Frank Le Curieux , Daniel Marzin

DOI: 10.1007/S11270-009-9981-3

关键词: Micronucleus testSoil contaminationBotanyGenotoxicityMicronucleusRepensHorticultureContaminationTrifolium repensVicia fabaBiologyEcological modellingEnvironmental engineeringPollutionWater Science and TechnologyEnvironmental chemistry

摘要: The DNA damage and cytotoxicity induced by CdCl2 solutions soils anthropogenically contaminated with heavy metals were studied using the micronucleus (MN) test. Vicia faba, a plant model widely used in liquid exposure assays, was adapted for direct to solid phase. In addition, MN assay new wild system, white clover (Trifolium repens). results obtained after confirmed that V. faba root cells sensitive revealed T. repens not appropriate detection of micronuclei (although they highly cytotoxic effect CdCl2). Concerning observed (solid-phase exposure), frequency scores increased statistically significant dose-related manner compared control plants. Regarding cells, this phase is use assay.

参考文章(37)
Te-Hsiu Ma, Tradescantia Micronuclei (Trad-MCN) Test For Environmental Clastogens In Vitro Toxicity Testing of Environmental Agents. pp. 191- 214 ,(1983) , 10.1007/978-1-4613-3566-5_10
B. A. Kihlman, Root Tips for Studying the Effects of Chemicals on Chromosomes Springer, Boston, MA. pp. 489- 514 ,(1971) , 10.1007/978-1-4615-8969-3_8
Nan Sang, Guangke Li, Genotoxicity of municipal landfill leachate on root tips of Vicia faba. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 560, pp. 159- 165 ,(2004) , 10.1016/J.MRGENTOX.2004.02.015
A. Marshak, The Effect of X-Rays on Chromosomes in Mitosis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 23, pp. 362- 369 ,(1937) , 10.1073/PNAS.23.7.362
Roberta Aina, Lucio Palin, Sandra Citterio, Molecular evidence for benzo[a]pyrene and naphthalene genotoxicity in Trifolium repens L. Chemosphere. ,vol. 65, pp. 666- 673 ,(2006) , 10.1016/J.CHEMOSPHERE.2006.01.071
Sandra Minissi, Enzo Lombi, Heavy metal content and mutagenic activity, evaluated by Vicia faba micronucleus test, of Tiber river sediments. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 393, pp. 17- 21 ,(1997) , 10.1016/S1383-5718(97)00093-4
Mingsheng Miao, Rongshu Fu, Dekui Yang, Liansheng Zheng, Vicia root micronucleus assay on the clastogenicity of water samples from the Xiaoqing River in Shandong Province of the People's Republic of China Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. ,vol. 426, pp. 143- 145 ,(1999) , 10.1016/S0027-5107(99)00057-3
Anna Fusconi, Cristina Gallo, Wanda Camusso, Effects of cadmium on root apical meristems of Pisum sativum L.: cell viability, cell proliferation and microtubule pattern as suitable markers for assessment of stress pollution. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 632, pp. 9- 19 ,(2007) , 10.1016/J.MRGENTOX.2007.03.012