Gene expression specificity of the mussel antifungal mytimycin (MytM)
作者： Molruedee Sonthi , Franck Cantet , Mylène Toubiana , Maria-Rosa Trapani , Maria-Giovanna Parisi
DOI: 10.1016/J.FSI.2011.10.017
关键词: Stimulation 、 Mytilin 、 Bacteria 、 Yeast 、 Candida albicans 、 Fusarium oxysporum 、 Microbiology 、 Gene expression 、 Biology 、 Myticin
摘要: Abstract We previously reported the nucleotide sequences and diversity of mytimycin (MytM) from Mediterranean mussel, Mytilus galloprovincialis. Using real-time PCR (q-PCR), we observed that MytM gene was mainly expressed in circulating hemocytes to a less extent mantle. In vivo challenge with bacteria or yeast, Candida albicans, did not increase expression as measured by q-PCR hemocytes. By contrast, injection filamentous fungus, Fusarium oxysporum, induced sudden strong at 9h p.i. (stimulation index 25.7 ± 2.1). Optimum stimulating dose 104 spores F. oxysporum per mussel. In same samples, AMP mytilin myticin showed no stimulation. Consequently, hypothesized existence 2 different signal transduction pathways, one activated other triggered fungi. A second performed 24 h after first an 3.5 ± 1.7). However, this significantly lower than first, suggesting efficient response rather significant protection.
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