Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis

摘要: Next generation sequencing (NGS) allows fast and massive production of both genome transcriptome sequence datasets. As the Mediterranean mussel Mytilus galloprovincialis is not available at present, we have explored possibility reducing whole efforts by using capture probes coupled with PCR amplification high-throughput 454-sequencing to enrich selected genomic regions. The enrichment DNA target sequences was validated real-time PCR, whereas efficacy applied strategy evaluated mapping 454-output reads against reference transcript data already for M. measuring coverage, SNPs, number de novo sequenced introns, complete gene sequences. Focusing on a size nearly 1.5 Mbp, obtained coverage which allowed identification more than 250 10,741 also This study confirms transcriptome-based gDNA regions as good expand knowledge specific subsets genes in nonmodel organisms.