Barbiturates decrease the expression of vascular endothelial growth factor in hypoxic cultures of porcine brain derived microvascular endothelial cells.
作者： S. Fischer , D. Renz , W. Schaper , G.F. Karliczek
DOI: 10.1016/S0169-328X(98)00171-5
关键词: Internal medicine 、 Cell type 、 Barbiturate 、 Biology 、 Autocrine signalling 、 Microcirculation 、 Endocrinology 、 Permeability (electromagnetism) 、 Vascular endothelial growth factor 、 Neuroprotection 、 Endothelial stem cell
摘要: Vascular endothelial growth factor (VEGF) is known to be produced in higher amounts during hypoxia by a variety of cell types and has been shown increase the permeability brain derived microvascular cells (BMEC) an autocrine mechanism. Because barbiturates, methohexital (MH) thiopental (TP), induced dose-dependent reduction hypoxia-induced changes BMEC, effect both barbiturates on VEGF expression was investigated. Both decreased BMEC concentration-dependent manner. This partly caused impairment mRNA stabilization. VEGF-induced normoxia were unaffected suggesting that MH TP are directly reducing synthesis. In conclusion, inhibiting these results monolayer hypoxia, which may contribute described neuroprotective action edema formation.
elsevier.com
sci-hub.se 参考文章(43)
Bradbury Mw, The structure and function of the blood-brain barrier. Federation proceedings. ,vol. 43, pp. 186- ,(1984)
D Lindholm, R Heumann, B Hengerer, H Thoenen, Interleukin 1 increases stability and transcription of mRNA encoding nerve growth factor in cultured rat fibroblasts. Journal of Biological Chemistry. ,vol. 263, pp. 16348- 16351 ,(1988) , 10.1016/S0021-9258(18)37599-9
F Malecaze, V Simorre-Pinatel, P Chollet, M Penary, M Guerrin, J Plouet, S Clamens, Vasculotropin-VEGF Stimulates Retinal Capillary Endothelial Cells Through an Autocrine Pathway Investigative Ophthalmology & Visual Science. ,vol. 35, pp. 3393- 3400 ,(1994)
D R Senger, L Van de Water, J Feder, H F Dvorak, D T Connolly, Purification and NH2-Terminal Amino Acid Sequence of Guinea Pig Tumor-secreted Vascular Permeability Factor Cancer Research. ,vol. 50, pp. 1774- 1778 ,(1990)
Motohiro Nomura, Sho-ichi Yamagishi, Shin-ichi Harada, Yasuhiko Hayashi, Tetsumori Yamashima, Junkoh Yamashita, Hiroshi Yamamoto, Possible participation of autocrine and paracrine vascular endothelial growth factors in hypoxia-induced proliferation of endothelial cells and pericytes. Journal of Biological Chemistry. ,vol. 270, pp. 28316- 28324 ,(1995) , 10.1074/JBC.270.47.28316
E. A. Pierce, R. L. Avery, E. D. Foley, L. P. Aiello, L. E. Smith, Vascular endothelial growth factor/vascular permeability factor expression in a mouse model of retinal neovascularization Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 905- 909 ,(1995) , 10.1073/PNAS.92.3.905
Michael D. O'Brien, Ischemic Cerebral Edema and the Blood-Brain Barrier: Distributions of Pertechnetate, Albumin, Sodium, and Antipyrine in Brains of Cats After Occlusion of the Middle Cerebral Artery JAMA Neurology. ,vol. 30, pp. 461- 465 ,(1974) , 10.1001/ARCHNEUR.1974.00490360037008
Søren-Peter Olesen, Rapid increase in blood-brain barrier permeability during severe hypoxia and metabolic inhibition. Brain Research. ,vol. 368, pp. 24- 29 ,(1986) , 10.1016/0006-8993(86)91038-3
Napoleone Ferrara, Keith A Houck, Lyn B Jakeman, Jane Winer, David W Leung, None, The vascular endothelial growth factor family of polypeptides. Journal of Cellular Biochemistry. ,vol. 47, pp. 211- 218 ,(1991) , 10.1002/JCB.240470305
Uwe Mischeck, J�rg Meyer, Hans-Joachim Galla, Characterization of γ-glutamyl transpeptidase activity of cultured endothelial cells from porcine brain capillaries Cell and Tissue Research. ,vol. 256, pp. 221- 226 ,(1989) , 10.1007/BF00224737