Chlamydia trachomatis vacuole maturation in infected macrophages.

摘要: Chlamydia trachomatis is an obligate intracellular bacterium responsible for one of the most common sexually transmitted diseases. In epithelial cells, C. resides in a modified membrane-bound vacuole known as inclusion, which isolated from endocytic pathway. However, maturation process within immune such macrophages, has not been studied extensively. Here, we demonstrated that RAW macrophages effectively suppressed growth and prevented Golgi stack disruption, hallmark defect cells after infection. Next, systematically examined association between various pathway markers. Spinning disk confocal time-lapse studies revealed significant rapid with Rab7 LAMP1, markers late endosomes lysosomes. Moreover, pretreatment inhibitor lysosome acidification led to increases macrophages. At later stages infection, associated autophagy marker LC3. TEM analysis confirmed portion resided double-membranebound compartments, characteristic autophagosomes. Together, these results suggest can suppress by targeting it rapidly lysosomes; moreover, activated at infection targets numbers invading bacteria, may enhance subsequent chlamydial antigen presentation. J. Leukoc. Biol. 92: 815–827; 2012.