Intracellular trafficking of Parachlamydia acanthamoebae.

摘要: Parachlamydia acanthamoebae is an obligate intracellular bacterium that naturally infects free-living amoebae. It a potential agent of pneumonia resists destruction by human macrophages. However, the strategy used this Chlamydia-like organism in order to resist macrophage unknown. We analysed trafficking P. within monocyte-derived Infected cells were immunolabelled for bacteria and various compartments using specific antibodies. colocalization with different subcellular epifluorescence confocal microscopy. Bacterial replication took place 4-6 h post infection acidic vacuoles. At time, colocalized Lamp-1, membrane marker late endosomal lysosomal compartments. A transient accumulation EEA1 15 min infection, rab7 mannose 6-phosphate receptor 30 confirmed traffic through endocytic pathway. The acquisition Lamp-1 was not after living heat-inactivated bacteria. 24.5% 79.5% acanthamoebae, respectively, vacuolar proton ATPase. Moreover, did cathepsin D, hydrolase, suggesting interferes maturation its vacuole. Thus, survives macrophages probably controlling vacuole biogenesis.