Studies on cellular recovery from injury. II. Ultrastructural studies on the recovery of the pars convoluta of the proximal tubule of the rate kidney from temporary ischemia.

摘要: The pars convoluta of the proximal tubule rat kidney was studied by light and electron microscopy during recovery phase from transient ischemia. left made ischemic clamping aorta just above renal artery leaving blood supply to right intestine intact. (P1 P2 segments) examined both immediately after various periods ischemia (15, 30, 60 120 min) same followed 3, 6, 12 24 h reflow (= phase). It found that for up min were compatible with cell survival whereas gave rise irreversible cellular changes. Before regaining a normal conformation phase, cells 15 characterized slightly decreased height, dispersed nuclear chromatin, mitochondria in orthodox increased numbers digestive vacuoles lipid droplets (stage A2). Most appeared when reflow. Cells 30 also stage A2 changes 3 remained this all studied. first passed into designated A1 then later A2. Stage condensed mitochondria, apparently sizes secondary lysosomes dilated rough surfaced endoplasmic reticulum; not survival. showed following alterations denoted as stages C D: shrunken pyknotic nuclei, swollen large flocculent densities, filled different sized vesicles apical portion C). D included which had undergone necrosis, i.e., phasma membranes organelles fragmented occurred debris lumens.