Comprehensive Two-Dimensional Liquid Chromatography with Stationary-Phase-Assisted Modulation Coupled to High-Resolution Mass Spectrometry Applied to Proteome Analysis of Saccharomyces cerevisiae
作者： Rudy J. Vonk , Andrea F. G. Gargano , Ekaterina Davydova , Henk L. Dekker , Sebastiaan Eeltink
DOI: 10.1021/ACS.ANALCHEM.5B00708
关键词: Mass spectrometry 、 Ion chromatography 、 Chemistry 、 Modulation 、 Shotgun proteomics 、 Dilution 、 Dimension (vector space) 、 Chromatography 、 Analytical chemistry 、 Column (database) 、 Volumetric flow rate
摘要: Stationary-phase-assisted modulation is used to overcome one of the limitations contemporary comprehensive two-dimensional liquid chromatography, which arises from combination a first-dimension column that typically narrow and long second-dimension wide short. Shallow gradients at low flow rates are applied in first dimension, whereas fast analyses (at high rates) required second dimension. Limitations this approach include sample capacity dilution complete system. Moreover, relatively for dimension make direct (splitless) hyphenation mass spectrometry difficult. In present study we demonstrate stationary-phase-assisted can be implemented an online LC (LC × LC) setup shift paradigm. The proposed active makes it possible choose virtually any first- ...
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