Peak refocusing using subsequent retentive trapping and strong eluent remobilization in liquid chromatography: A theoretical optimization study

摘要: The present paper presents the mathematical expressions for concentration enhancement which can be expected when applying a subsequent retentive trapping and strong eluent remobilization process in packed trap column connected to end of an analytical separation column. established optimal loading times, dimensions are illustrated confirmed using numerical simulations release process. These also provide direct insight how bands deformed sharpened during different steps simulations, as well expressions, example show that backward elution mode time should exceed minimum value allow front fully overtake remobilized band this is necessary condition get completely peak (in both forward modes). it very critical occurs close possible exit, most sensitive broadening once they have been sharpened. refocusing traps hence filled with particles producing smallest plate heights, detector short small i.d. connection tubing. It was found that, if there would no trap, achievable same either or weakly retaining trap. true advantage high retention leads volumes, turn minimizes distance has travel reach detector. This then (inevitable practice) helps keeping enhancement.