In-house reference (IHR) preparation of Candida albicans allergen extract. A standardized extraction procedure.
作者： J. Savolainen , K. Kalimo , R. Einarsson , A. Koivikko , M. Viander
DOI: 10.1111/J.1398-9995.1998.TB03906.X
关键词: Crossed radioimmunoelectrophoresis 、 Corpus albicans 、 Allergen extract 、 Extraction (chemistry) 、 In vivo 、 Candida albicans 、 Allergen 、 Coefficient of variation 、 Biology 、 Chromatography 、 Microbiology
摘要: A standardized, controlled procedure for preparation of an in-house reference (IHR) allergen extract Candida albicans is described. The procedure, based on previous studies allergens C. albicans, designed to yield a maximum in optimum extraction conditions and provide further production. SDS-PAGE, IgE-immunoblotting, crossed radioimmunoelectrophoresis (CRIE) analyses showed that the reproducible with acceptable batch-to-batch variation. variation content most important allergens, namely, proteins molecular weights 46, 29, 27 kDa pooled final batches, (coeff. < 15%), although intermediate batches different strains, coefficient may occasionally exceed 20%. comparison other preparations used our also presented. resulting can be as production experimental vitro vivo studies.
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