Rapid screening of tissue microarrays for Her-2 fluorescence in situ hybridization testing is an accurate, efficient and economic method of providing an entirely in situ hybridization-based Her-2 testing service.

摘要: Aim:  Fluorescence in situ hybridization (FISH) testing is the ‘gold standard’ method for Her-2 status assessment breast cancer patients, yet only employed about 30% of tests carried out because cost and labour considerations. We have previously described tissue microarray (TMA)-based to eliminate constraints, now describe a rapid screening approach reduce time spent testing. Methods results:  examined 88 cases invasive on TMAs comparing formal FISH scoring with technique. Each core was screened by two observers results recorded as positive, equivocal or negative. timed. Data were analysed counts. Using screening, two-thirds negative half positive could be eliminated 100% accuracy. It took 2 min per observer case screen six TMA cores at ×100 magnification. The remaining required counting, which no longer than whole-section techniques. Conclusion:  Rapid routine safe, economical efficient. technique ensures that all patients receive testing.