Glycomodification and characterization of anti-colorectal cancer immunotherapeutic monoclonal antibodies in transgenic tobacco
作者： Yangkang So , Kyung-Jin Lee , Deuk-Su Kim , Jeong-Hwan Lee , Doo-Byoung Oh
DOI: 10.1007/S11240-012-0249-Z
关键词: Receptor 、 Biology 、 Glycosylation 、 Monoclonal antibody 、 Antigen 、 CD64 、 KDEL 、 Endoplasmic reticulum 、 Antibody-dependent cell-mediated cytotoxicity 、 Molecular biology
摘要: Anti-colorectal cancer mAb CO17-1A (IgG2a) recognizes the antigen GA733, which is highly expressed on surface membrane of human colorectal carcinoma cells. In this study, a transgenic tobacco system for production was developed. The construct included KDEL sequence, an endoplasmic reticulum (ER) retention signal attached to C-terminus heavy chain, target accumulation into ER. An immunoblot showed significantly enhanced levels expression plant-derived mAbK (mAbPK) compared mAbP without sequence. ELISA assay using cells confirmed that mAbPK also higher than mAbP. Glycosylation analysis revealed had plant-specific glycans; whereas, primarily oligomannose glycans. FACS Fc domains both and mammalian-derived (mAbM) similar binding activity FcγRI receptor (CD64). However, slightly lower mAbM. antibody-dependent cell cytotoxicity mAbPK, against cells, as efficient mAbM; whereas very low. These results suggest localized accumulated in ER eventually with glycan anti-cancer biological parental
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