An investigation of aldehyde fuchsin staining of unoxidized insulin

摘要: Aldehyde fuchsin is a standard stain for the secretion granules of pancreatic B cells. The participation either insulin or proinsulin in aldehyde staining dispute. There some evidence that permanganate oxidized stained by fuchsin. unoxidized has not been investigated adequately despite excellent results with tissue sections. Unoxidized and suspended electrophoresis polyacrylamide gels were fixed Bouin's fluid placed one hour. Because proteins fuchsin, it was concluded neither nor are responsible intense cell A series controlled experiments undertaken to test effects fixatives, oxidation destaining procedures on insulin, other immobilized gels. It demonstrated only cystine content had no apparent relation staining. likely be