Animal, fungi, and plant genome sequences harbour different non-canonical splice sites

摘要: Abstract Most protein encoding genes in eukaryotes contain introns which are interwoven with exons. After transcription, need to be removed order generate the final mRNA can translated into an amino acid sequence. Precise excision of by spliceosome requires conserved dinucleotides mark splice sites. However, there variations highly combination GT at 5’ end and AG 3’ intron genome. GC-AG AT-AC two major non-canonical site combinations have been known for years. During last years, various minor were detected numerous dinucleotide permutations. Here we expand systematic investigations plants all analysing fungal animal genome sequences. Comparisons between these three kingdoms revealed several differences such as a substantially increased CT-AC frequency Canonical GT-AG antisense transcripts could one explanation this observation. In addition, high numbers GA-AG observed Eurytemora affinis Oikopleura dioica. A variant U1 snRNA isoform might allow recognition GA site. depth investigation usage based on RNA-Seq read mappings indicates generally higher flexibility compared across animals, fungi, plants.