High-Throughput Screening for Isolation of Enhanced Green Fluorescent Protein Expressing Transformants of Filamentous Fungus Using an Image Analyzer
作者： Satoshi Suzuki , Hiroko Taketani , Ken-Ichi Kusumoto , Yutaka Kashiwagi
DOI: 10.1016/S1389-1723(03)80201-3
关键词: Green fluorescent protein 、 Spectrum analyzer 、 Quantitative analysis (chemistry) 、 Biology 、 Reporter gene 、 Aspergillus oryzae 、 Molecular biology 、 Isolation (microbiology) 、 Fluorescence 、 High-throughput screening
摘要: High-throughput screening of enhanced green fluorescent protein (EGFP) gene-trap transformants filamentous fungus was achieved for the first time using an image analyzer to measure their intensity. For quantitative analysis EGFP intensity per unit cell mass, we developed a method measurement mass dye SP-Dil. This offers effective and convenient tool expressing as reporter, fungal at jig levels.
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