High throughput transfection of filamentous fungi

作者: Bianca Elisabeth Maria Gielesen , Adriana Marina Riemens , Marco Alexander Van Den Berg

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摘要: The present invention provides a method for the transfection of filamentous fungal cells, comprising providing multitude containers, filling into each container an amount polymer needed transfection, cells to be transfected as well aqueous solution reagent incubating resulting mixture, removing from incubated mixture; and selecting which have been transformed, characterized in that total volume mixture is less than 1 ml per container. Furthermore, use transformed production proteins or metabolites.

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