Microbial strain improvement by a htp genomic engineering platform
作者： Kenneth S. Bruno , Jed Dean , Kasia Gora , Michael Flashman , Erin Shellman
DOI:
关键词: Computational biology 、 Computer science 、 Microbial host 、 Strain (biology) 、 Host (network) 、 Automation 、 Genomic engineering 、 Pattern recognition (psychology) 、 Genetic design
摘要: The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, advanced machine learning protocols. This integrative utilizes suite of tool sets to create genetic design libraries, which are derived from, inter alia, scientific insight iterative pattern recognition. described herein strain host agnostic therefore can be implemented across taxa. Furthermore, the disclosed modulate or improve any parameter interest.
lens.org 参考文章(365)
Pavla Vašicová, Miroslav Pátek, Jan Nešvera, Hermann Sahm, Bernhard Eikmanns, Analysis of the Corynebacterium glutamicum dapA promoter. Journal of Bacteriology. ,vol. 181, pp. 6188- 6191 ,(1999) , 10.1128/JB.181.19.6188-6191.1999
Yukihiro Nakajo, Sadao Isshiki, Yasuhiko Takeda, Mikio Fujii, Plasmid containing a gene for tetracycline resistance and DNA fragments derived therefrom ,(1990)
Robert W. Rosenstein, Solid phase chromatographic immunoassay ,(1993)
Hideaki Yukawa, Masayuki Inui, DNA fragment having promoter function ,(2005)
Simon Fischer, René Handrick, Kerstin Otte, The art of CHO cell engineering: A comprehensive retrospect and future perspectives Biotechnology Advances. ,vol. 33, pp. 1878- 1896 ,(2015) , 10.1016/J.BIOTECHADV.2015.10.015
Elaine A. Best, Christopher C. Frye, Charles L. Hershberger, Methods of reducing the levels of protoporphyrin IX in recombinant hemoglobin preparations ,(1997)
Michael D. Lynch, Tanya E. W. Lipscomb, T Gill Ryan, Mixed-library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes ,(2011)
Willem Peter Christiaan Stemmer, Robert J. Lipshutz, End-complementary polymerase reaction ,(1996)
Willem P. C. Stemmer, Jeremy Minshull, Methods and compositions for cellular and metabolic engineering ,(1997)
B Möckel, L Eggeling, H Sahm, Functional and structural analyses of threonine dehydratase from Corynebacterium glutamicum. Journal of Bacteriology. ,vol. 174, pp. 8065- 8072 ,(1992) , 10.1128/JB.174.24.8065-8072.1992