A 470 bp WAP-promoter fragment confers lactation independent, progesterone regulated mammary-specific gene expression in transgenic mice.

摘要: The ability of a 470 bp sub-fragment the murine whey acidic protein (WAP) promoter in context retroviral expression plasmid to direct gene mammary epithelial cells was analysed number independent transgenic mouse lines. In contrast previous findings with genuine 2.5 kb fragment, our studies revealed highly gland-specific detectable only non-lactating animals. This suggested mainly progesterone-regulated activity short fragment. Therefore, transgene examined progesterone-determined estrous cycle and during pregnancy. accordance vitro data from stably transfected cell lines, both situations upregulated at stages associated high progesterone levels. Taken together these provide deeper insight into WAP-promoter regulation stress usefulness shortened fragment for lactation mammary-targeted expression.