Paper-based immunoaffinity devices for accessible isolation and characterization of extracellular vesicles

摘要: We present a paper-based immunoaffinity platform for separating subsets of extracellular vesicles (EVs). This method is inexpensive, robust, easy-to-use (easy-to-prepare), and compatible with downstream analyses, such as scanning electron microscopy (SEM), enzyme-linked immunosorbent assays (ELISA), transcriptome analysis. EVs are small membranous shed from both healthy diseased cells contain nucleic acid protein cargo. have been increasingly recognized means cell–cell communication hold great potential clinical applications. However, current protocols isolation often lengthy, cumbersome require expensive equipment. Here, we isolated volumes human serum aqueous humor samples using devices. Captured were analyzed morphologically SEM appeared statistically different in size (p value < 2.4 × 10−22) circularity 3.6 10−9) between bearing surface markers. Assessing the amount captured ELISA antibodies conjugated to horseradish peroxidase produce colorimetric readout was accomplished within 10 min. RNAs contained can be extracted provide information disease management. These devices, believe, open opportunities wide range applications basic biology medicine.