Rapid Identification of Linear Protein Domain Binding Motifs Using Peptide SPOT Arrays

摘要: Understanding protein-protein interactions is a key step in unravelling the roles proteins play cellular function. The ability to analyse rapidly and economically powerful research tool. Using peptide SPOT arrays, peptides of known sequence can be synthesized directly discrete spots on cellulose membrane assayed for an interaction with protein interest. Several hundred each membrane; therefore, this method amenable designing high-throughput binding studies. arrays are particularly well suited deducing peptidic motifs within that difficult purify sufficient quantities traditional biochemical analyses, as determining specificities targets undefined Peptide have been used extensively define surfaces. In chapter, we will outline steps involved probing array identify