Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support
作者： Ronald Frank
DOI: 10.1016/S0040-4020(01)85612-X
关键词: Peptide synthesis 、 Cellulose 、 Combinatorial chemistry 、 Membrane 、 Chemistry 、 Spot synthesis 、 Peptide 、 Chromatography 、 Peptide array 、 Amino acid 、 Chemical synthesis
摘要: Abstract Simultaneous parallel syntheses at distinct positions on a membrane support is exemplified with the preparation of series predefined, short peptide sequences (1). Cellulose paper sheets are used as absorptive membranes. Peptides assembled by manual or automated spotting small aliquots solutions containing activated amino acid derivatives onto marked sheets. The application this method to rapid epitope analysis demonstrated.
elsevier.com
sci-hub.se 参考文章(15)
K. K. Stanley, J. Herz, Topological mapping of complement component C9 by recombinant DNA techniques suggests a novel mechanism for its insertion into target membranes. The EMBO Journal. ,vol. 6, pp. 1951- 1957 ,(1987) , 10.1002/J.1460-2075.1987.TB02457.X
M. Zouhair ATASSI, Antigenic structures of proteins FEBS Journal. ,vol. 145, pp. 1- 20 ,(1984) , 10.1111/J.1432-1033.1984.TB08516.X
E. Getzoff, H. Geysen, S. Rodda, H Alexander, J. Tainer, R. Lerner, Mechanisms of antibody binding to a protein Science. ,vol. 235, pp. 1191- 1196 ,(1987) , 10.1126/SCIENCE.3823879
J. Devlin, L. Panganiban, P. Devlin, Random peptide libraries: a source of specific protein binding molecules. Science. ,vol. 249, pp. 404- 406 ,(1990) , 10.1126/SCIENCE.2143033
J. Scott, G. Smith, Searching for Peptide Ligands With an Epitope Library Science. ,vol. 249, pp. 386- 390 ,(1990) , 10.1126/SCIENCE.1696028
H. Geysen, J. Tainer, S. Rodda, T. Mason, H Alexander, E. Getzoff, R. Lerner, Chemistry of antibody binding to a protein Science. ,vol. 235, pp. 1184- 1190 ,(1987) , 10.1126/SCIENCE.3823878
Ronald Frank, Renate Döring, Simultaneous multiple peptide synthesis under continuous flow conditions on cellulose paper discs as segmental solid supports Tetrahedron. ,vol. 44, pp. 6031- 6040 ,(1988) , 10.1016/S0040-4020(01)89791-X
Andrew M. Bray, N. Joe Maeji, H. Mario Geysen, The simultaneous multiple production of solution phase peptides; assessment of the geysen method of simultaneous peptide synthesis. Tetrahedron Letters. ,vol. 31, pp. 5811- 5814 ,(1990) , 10.1016/S0040-4039(00)97966-8
Viktor Krchňák, Josef Vágner, Pavel Šafář, Michal Lebl, Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicator Collection of Czechoslovak Chemical Communications. ,vol. 53, pp. 2542- 2548 ,(1988) , 10.1135/CCCC19882542
GREGG B. FIELDS, RICHARD L. NOBLE, Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids International Journal of Peptide and Protein Research. ,vol. 35, pp. 161- 214 ,(2009) , 10.1111/J.1399-3011.1990.TB00939.X