SPOTing Acetyl-Lysine Dependent Interactions

摘要: Post translational modifications have been recognized as chemical signals that create docking sites for evolutionary conserved effector modules, allowing signal integration within large networks of interactions. Lysine acetylation in particular has attracted attention a regulatory modification, affecting chromatin structure and linking to transcriptional activation. Advances peptide array technologies facilitated the study acetyl-lysine-containing linear motifs interacting with bromodomain module, which specifically recognizes binds acetylated sequences histones other proteins. Here we summarize recent work employing SPOT technology identify acetyl-lysine dependent interactions document protocols adapted our lab, well efforts characterize such bromodomain-histone Our results highlight versatility methods establish an affordable tool rapid access potential protein/modified-peptide involving lysine acetylation.